Publications: Prof Richard Pickersgill
Gu S, Bradley-Clarke J, Rose R-S, Warren MJ, Pickersgill RW
(
2024
)
.
Enzyme-cargo encapsulation peptides bind between tessellating tiles of the bacterial microcompartment shell
.
Journal of Biological Chemistry
vol.
300
,
(
6
)
Zhang S, Gu S, Rycroft P, Ruaudel F, Delolme F, Robert X, Ballut L, Pickersgill RW et al.
(
2022
)
.
Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall
.
mBioe0025322
-
e0025322
.
Robledo Retana T, Bradley-Clarke J, Croll T, Rose R, Stagg AJ, Villalba M, Honti I, Pickersgill RW
(
2020
)
.
Lig v 1 structure and the inflammatory response to the Ole e 1 protein family
.
Allergy
Conti D, Gul P, Islam A, Martín-Durán JM, Pickersgill RW, Draviam VM
(
2019
)
.
Kinetochores attached to microtubule-ends are stabilised by astrin bound pp1 to ensure proper chromosome segregation
.
eLife
vol.
8
,
UDDIN I, PICKERSGILL R, Gosal W
(
2019
)
.
Structure of Nanotubes Formed From Bacterial Microcompartment Shell Proteins
.
Lee MJ, Mantell J, Brown IR, Fletcher JM, Verkade P, Pickersgill RW, Woolfson DN, Frank S et al.
(
2018
)
.
De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments
.
Nature Communications
vol.
9
,
(
1
)
Pluta P, Roversi P, Bernardo-Seisdedos G, Rojas AL, Cooper JB, Gu S, Pickersgill RW, Millet O
(
2018
)
.
Structural basis of pyrrole polymerization in human porphobilinogen deaminase
.
Biochim Biophys Acta
Uddin I, Frank S, Warren MJ, Pickersgill RW
(
2018
)
.
A Generic Self-Assembly Process in Microcompartments and Synthetic Protein Nanotubes
.
Small
vol.
14
,
(
19
)
Uddin I, Frank S, Warren MJ, Pickersgill RW
(
2018
)
.
Synthetic Protein Nanotubes: A Generic Self‐Assembly Process in Microcompartments and Synthetic Protein Nanotubes (Small 19/2018)
.
Small
vol.
14
,
(
19
)
Abbott W, Alber O, Bayer E, Berrin J-G, Boraston A, Brumer H, Brzezinski R, Clarke A et al.
(
2017
)
.
Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes
.
GLYCOBIOLOGY
vol.
28
,
(
1
)
3
-
8
.
Tabaee Damavandi P, Dove MT, Pickersgill RW
(
2017
)
.
A review of drug therapy for sporadic fatal insomnia
.
Prion
vol.
11
,
(
5
)
293
-
299
.
Gu S, Vladimir VE, Shaw R, Pickersgill, Garnett JA
(
2017
)
.
The role of intrinsic disorder and dynamics in the assembly and function of the type II secretion system
.
BBA - Proteins and Proteomics
Qui J, Zeng Y, Pickersgill R, Donnan R, Sushko O, Yang B
(
2017
)
.
Introducing Quasi-Optical Terahertz Circular Dichroism Spectroscopy
.
Conference:
2017 XI International Conference on Antenna Theory and Techniques (ICATT)26
-
29
.
Gu S, Sushko O, Deery E, Warren MJ, Pickersgill RW
(
2015
)
.
Crystal structure of CobK reveals strand-swapping between Rossmann-fold domains and molecular basis of the reduced precorrin product trap
.
Scientific Reports
vol.
5
,
(
1
)
Ali S, Søndergaard CR, Teixeira S, Pickersgill RW
(
2015
)
.
Structural insights into the loss of catalytic competence in pectate lyase activity at low pH
.
FEBS Letters
vol.
589
,
(
21
)
3242
-
3246
.
Sushko O, Qiu J, Dubrovka R, Pickersgill RW, Donnan RS
(
2015
)
.
Sub-Terahertz Spectroscopy as a Probe for Protein Stability in an Ionic Environment
.
Conference:
2015 40th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz)1
-
2
.
Pang A, Frank S, Brown I, Warren MJ, Pickersgill RW
(
2014
)
.
Structural insights into higher order assembly and function of the bacterial microcompartment protein PduA
.
J Biol Chem
vol.
289
,
(
32
)
22377
-
22384
.
Lawrence AD, Taylor SL, Scott A, Rowe ML, Johnson CM, Rigby SEJ, Geeves MA, Pickersgill RW et al.
(
2014
)
.
FAD binding, cobinamide binding and active site communication in the corrin reductase (CobR)
.
Biosci Rep
vol.
34
,
(
4
)
Palmer DJ, Schroeder S, Lawrence AD, Deery E, Lobo SA, Saraiva LM, McLean KJ, Munro AW et al.
(
2014
)
.
The structure, function and properties of sirohaem decarboxylase - an enzyme with structural homology to a transcription factor family that is part of the alternative haem biosynthesis pathway
.
MOLECULAR MICROBIOLOGY
vol.
93
,
(
2
)
247
-
261
.
Terali K, Beavil RL, Pickersgill RW, van der Giezen M
(
2013
)
.
The effect of the adaptor protein Isd11 on the quaternary structure of the eukaryotic cysteine desulphurase Nfs1
.
Biochem Biophys Res Commun
vol.
440
,
(
2
)
235
-
240
.
Gu S, Rehman S, Pickersgill RW
(
2013
)
.
Crystal structure of the bacterial type II secretion system secretin/pilotin complex
.
Acta Crystallographica Section A: Foundations and advances
.
vol.
69
,
s351
-
s351
.
Rehman S, Gu S, Shevchik VE, Pickersgill RW
(
2013
)
.
Anatomy of secretin binding to the Dickeya dadantii type II secretion system pilotin
.
Acta Crystallogr D Biol Crystallogr
vol.
69
,
(
Pt 8
)
1381
-
1386
.
DONNAN RS, Pickersgill R, Krauss N, Yang B, Sushko O
(
2013
)
.
Lagrangian dynamics analysis of sub-THz skeletal absorption spectra of amino acids acquired by vector network analysis
.
Chemical Physics Letters
vol.
558
,
104
-
108
.
Pang A, Liang M, Prentice MB, Pickersgill RW
(
2012
)
.
Substrate channels revealed in the trimeric Lactobacillus reuteri bacterial microcompartment shell protein PduB
.
Acta Crystallogr D Biol Crystallogr
vol.
68
,
(
Pt 12
)
1642
-
1652
.
Ali S, Blakeley M, Søndergaard CR, Pellegrini E, Teixeira SCM, Pickersgill RW
(
2012
)
.
A joint neutron/X-ray crystallographic study on the mechanism of pectate lyase
.
Acta Crystallographica Section A: Foundations and advances
.
vol.
68
,
s91
-
s91
.
Gu S, Kelly G, Wang X, Frenkiel T, Shevchik VE, Pickersgill RW
(
2012
)
.
Solution structure of homology region (HR) domain of type II secretion system
.
J Biol Chem
vol.
287
,
(
12
)
9072
-
9080
.
Gu S, Rehman S, Wang X, Shevchik VE, Pickersgill RW
(
2012
)
.
Structural and functional insights into the pilotin-secretin complex of the type II secretion system
.
PLoS Pathog
vol.
8
,
(
2
)
Deery E, Schroeder S, Lawrence AD, Taylor SL, Seyedarabi A, Waterman J, Wilson KS, Brown D et al.
(
2012
)
.
An enzyme-trap approach allows isolation of intermediates in cobalamin biosynthesis
.
NATURE CHEMICAL BIOLOGY
vol.
8
,
(
11
)
933
-
940
.
Wang X, Pineau C, Gu S, Guschinskaya N, Pickersgill RW, Shevchik VE
(
2012
)
.
Cysteine Scanning Mutagenesis and Disulfide Mapping Analysis of Arrangement of GspC and GspD Protomers within the Type 2 Secretion System
.
JOURNAL OF BIOLOGICAL CHEMISTRY
vol.
287
,
(
23
)
19082
-
19093
.
Martin JE, Chowdhury ATMD, McElwaine S, Fikree A, Minassian B, Broad J, Knowles CH, Allen L et al.
(
2012
)
.
Intestinal Glycogen Storage Disease, and the Molecular Basis of Pseudo-obstruction with Polyglucosan Bodies
.
JOURNAL OF PATHOLOGY
.
vol.
226
,
S24
-
S24
.
Pickersgill RW
(
2012
)
.
Pathology: A Structural Biologist's View
.
JOURNAL OF PATHOLOGY
.
vol.
226
,
S7
-
S7
.
Ali S, Texeira S, Pickersgill RW
(
2011
)
.
Joint neutron/X-ray crystallographic study on the mechanism of pectate lyase
.
Acta Crystallographica Section A: Foundations and advances
.
vol.
67
,
c734
-
c734
.
Martin JE, Chowdhury AH, McElwaine S, Fikree A, Broad J, Knowles CH, Allen L, Bevan S et al.
(
2011
)
.
A Novel Phenotype of Glycogen Storage Disease Presenting as Gastrointestinal Neuromuscular Disease
.
Gastroenterology
vol.
140
,
(
5
)
s
-
705
.
Pang A, Warren MJ, Pickersgill RW
(
2011
)
.
Structure of PduT, a trimeric bacterial microcompartment protein with a 4Fe-4S cluster-binding site
.
Acta Crystallogr D Biol Crystallogr
vol.
67
,
(
Pt 2
)
91
-
96
.
Romao CV, Ladakis D, Lobo SAL, Carrondo MA, Brindley AA, Deery E, Matias PM, Pickersgill RW et al.
(
2011
)
.
Evolution in a family of chelatases facilitated by the introduction of active site asymmetry and protein oligomerization
.
P NATL ACAD SCI USA
vol.
108
,
(
1
)
97
-
102
.
Martin JE, Chowdhury AH, McElwaine S, Fikree A, Broad J, Knowles CH, Allen L, Bevan S et al.
(
2011
)
.
A Novel Phenotype of Glycogen Storage Disease Presenting as Gastrointestinal Neuromuscular Disease
.
GASTROENTEROLOGY
.
vol.
140
,
S705
-
S705
.
Brocklehurst K, Gul S, Pickersgill RW
(
2010
)
.
Substrate Recognition
.
Amino Acids, Peptides and Proteins in Organic Chemistry
,
Wiley
Seyedarabi A, Hutchison T, To TT, Deery E, Brindley A, Warren MJ, Pickersgill RW
(
2010
)
.
Cloning, purification and preliminary crystallographic analysis of cobalamin methyltransferases from Rhodobacter capsulatus
.
Acta Crystallogr Sect F Struct Biol Cryst Commun
vol.
66
,
(
Pt 12
)
1652
-
1656
.
Seyedarabi A, Sullivan JA, Sasakawa C, Pickersgill RW
(
2010
)
.
A disulfide driven domain swap switches off the activity of Shigella IpaH9.8 E3 ligase
.
FEBS Lett
vol.
584
,
(
19
)
4163
-
4168
.
Shala K, Yang B, TT T, Pickersgill RW, Krauss N, Donnan RS
(
2010
)
.
Millimeter wave spectroscopy of glucose and a calcium-binding protein using a vector network analyser
.
Conference:
35th IRMMW-THZ Internation Conference, 2010
(
Rome, Italy
)
from:
05/09/2010
to:
10/09/2010
,
1
-
2
.
Login FH, Fries M, Wang XH, Pickersgill RW, Shevchik VE
(
2010
)
.
A 20-residue peptide of the inner membrane protein OutC mediates interaction with two distinct sites of the outer membrane secretin OutD and is essential for the functional type II secretion system in Erwinia chrysanthemi
.
Molecular Microbiology
vol.
76
,
(
4
)
944
-
955
.
Seyedarabi A, To TT, Ali S, Hussain S, Fries M, Madsen R, Clausen MH, Teixteira S et al.
(
2010
)
.
Structural insights into substrate specificity and the anti beta-elimination mechanism of pectate lyase
.
Biochemistry
vol.
49
,
(
3
)
539
-
546
.
Heldt D, Frank S, Seyedarabi A, Ladikis D, Parsons JB, Warren MJ, Pickersgill RW
(
2009
)
.
Structure of a trimeric bacterial microcompartment shell protein, EtuB, associated with ethanol utilization in Clostridium kluyveri
.
BIOCHEM J
vol.
423
,
199
-
207
.
Brindley AA, Pickersgill RW, Partridge JC, Dunstan DJ, Hunt DM, Warren MJ
(
2008
)
.
Enzyme sequence and its relationship to hyperbaric stability of artificial and natural fish lactate dehydrogenases
.
PLoS One
vol.
3
,
(
4
)
Lawrence AD, Deery E, McLean KJ, Munro AW, Pickersgill RW, Rigby SEJ, Warren MJ
(
2008
)
.
Identification, characterization, and structure/function analysis of a corrin reductase involved in adenosylcobalamin biosynthesis
.
J BIOL CHEM
vol.
283
,
(
16
)
10813
-
10821
.
Jenkins J, Scott M, Mayans O, Pickersgill R, Harris G, Connerton I, Gravesen T
(
2007
)
.
Crystallization and preliminary X‐ray analysis of pectin lyase A from Aspergillus niger
.
Acta Crystallographica Section D, Structural Biology
vol.
52
,
(
2
)
402
-
404
.
Lo Leggio L, Parry NJ, Van Beeumen J, Claeyssens M, Bhat MK, Pickersgill RW
(
2007
)
.
Crystallization and preliminary X‐ray analysis of the major endoglucanase from Thermoascus aurantiacus
.
Acta Crystallographica Section D, Structural Biology
vol.
53
,
(
5
)
599
-
604
.
Harris GW, Jenkins JA, Connerton I, Pickersgill RW
(
2007
)
.
Refined Crystal Structure of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens at 1.8 Å Resolution
.
Acta Crystallographica Section D, Structural Biology
vol.
52
,
(
2
)
393
-
401
.
Pickersgill RW, Rizkallah P, Harris GW, Goodenough PW
(
2007
)
.
Determination of the structure of papaya protease omega
.
Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials
vol.
47
,
(
5
)
766
-
771
.
Pickersgill RW
(
2007
)
.
One‐dimensional disorder in spinach ribulose bisphosphate carboxylase crystals
.
Acta Crystallographica Section A: Foundations and advances
vol.
43
,
(
4
)
502
-
506
.
Fries M, Ihrig J, Brocklehurst K, Shevchik VE, Pickersgill RW
(
2007
)
.
Molecular basis of the activity of the phytopathogen pectin methylesterase
.
EMBO J
vol.
26
,
(
17
)
3879
-
3887
.
Frank S, Deery E, Brindley AA, Leech HK, Lawrence A, Heathcote P, Schubert HL, Brocklehurst K et al.
(
2007
)
.
Elucidation of substrate specificity in the cobalamin (vitamin B12) biosynthetic methyltransferases. Structure and function of the C20 methyltransferase (CbiL) from Methanothermobacter thermautotrophicus
.
J Biol Chem
vol.
282
,
(
33
)
23957
-
23969
.
Pickersgill RW
(
2007
)
.
Design and engineering of hydrolytic enzymes for enhanced performance in the agricultural and food industries
.
Journal of Chemical Technology & Biotechnology
vol.
57
,
(
3
)
290
-
291
.
Ooi A, Hussain S, Seyedarabi A, Pickersgill RW
(
2006
)
.
Structure of internalin C from Listeria monocytogenes
.
Acta Crystallogr D Biol Crystallogr
vol.
62
,
(
Pt 11
)
1287
-
1293
.
Opaleye O, Rose R-S, Whittaker MM, Woo E-J, Whittaker JW, Pickersgill RW
(
2006
)
.
Structural and spectroscopic studies shed light on the mechanism of oxalate oxidase
.
J Biol Chem
vol.
281
,
(
10
)
6428
-
6433
.
Graham CE, Brocklehurst K, Pickersgill RW, Warren MJ
(
2006
)
.
Characterization of retinaldehyde dehydrogenase 3
.
Biochem J
vol.
394
,
(
Pt 1
)
67
-
75
.
Frank S, Brindley AA, Deery E, Heathcote P, Lawrence AD, Leech HK, Pickersgill RW, Warren MJ
(
2005
)
.
Anaerobic synthesis of vitamin B12: characterization of the early steps in the pathway
.
Biochem Soc Trans
vol.
33
,
(
Pt 4
)
811
-
814
.
Okuda J, Toyotome T, Kataoka N, Ohno M, Abe H, Shimura Y, Seyedarabi A, Pickersgill R et al.
(
2005
)
.
Shigella effector IpaH9.8 binds to a splicing factor U2AF(35) to modulate host immune responses
.
BIOCHEM BIOPH RES CO
vol.
333
,
(
2
)
531
-
539
.
Pickersgill RW, Harris GW, Garman E
(
2005
)
.
Structure of monoclinic papain at 1.60 Å resolution
.
Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials
vol.
48
,
(
1
)
59
-
67
.
Harris GW, Pickersgill RW, Howlin B, Moss DS
(
2005
)
.
The segmented anisotropic refinement of monoclinic papain by the application of the rigid‐body TLS model and comparison to bovine ribonuclease A
.
Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials
vol.
48
,
(
1
)
67
-
75
.
SUMNER IG, HARRIS GW, TAYLOR MAJ, PICKERSGILL RW, OWEN AJ, GOODENOUGH PW
(
2005
)
.
Factors effecting the thermostability of cysteine proteinases from Carica papaya
.
The FEBS Journal
vol.
214
,
(
1
)
129
-
134
.
PICKERSGILL RW, SUMNER IG, GOODENOUGH PW
(
2005
)
.
Preliminary crystallographic data for protease Ω
.
The FEBS Journal
vol.
190
,
(
2
)
443
-
444
.
Jenkins J, Shevchik VE, Hugouvieux-Cotte-Pattat N, Pickersgill RW
(
2004
)
.
The crystal structure of pectate lyase Pel9A from Erwinia chrysanthemi
.
J BIOL CHEM
vol.
279
,
(
10
)
9139
-
9145
.
Pickersgill RW
(
2003
)
.
A primordial structure underlying amyloid
.
STRUCTURE
vol.
11
,
(
2
)
137
-
138
.
PICKERSGILL RW, Jenkins JA
(
2003
)
.
The structures and active sites of pectinases
.
Advances in pectin and pectinase research
.
Editors:
Voragen, AGJ, Voragen, F, Schols, H, Visser, R et al.
,
Conference:
International symposium on pectins and pectinases (2nd)
(
Rotterdam, The Netherlands
)
from:
05/2001
to:
05/2001
,
267
-
278
.
Pickersgill RW
(
2002
)
.
Complex cell signaling molecules from ancient molecular glue
.
STRUCTURE
vol.
10
,
(
10
)
1287
-
1288
.
Woo EJ, Marshall J, Bauly J, Chen JG, Venis M, Napier RM, Pickersgill RW
(
2002
)
.
Crystal structure of auxin-binding protein 1 in complex with auxin
.
EMBO J
vol.
21
,
(
12
)
2877
-
2885
.
Loris R, Steyaert J, Maes D, Lisgarten J, Pickersgill R, Wyns L
(
2002
)
.
Crystal structure determination and refinement at 2.3-A resolution of the lentil lectin
.
Biochemistry
vol.
32
,
(
51
)
Bhat MK, Pickersgill RW, Perry BN, Brown RA, Jones ST, Mueller-Harvey I, Sumner IG, Goodenough PW
(
2002
)
.
Modification of the head-group selectivity of porcine pancreatic phospholipase A2 by protein engineering
.
Biochemistry
vol.
32
,
(
45
)
12203
-
12208
.
Palomäki T, Pickersgill R, Riekki R, Romantschuk M, Saarilahti HT
(
2002
)
.
A putative three-dimensional targeting motif of polygalacturonase (PehA), a protein secreted through the type II (GSP) pathway in Erwinia carotovora
.
Mol Microbiol
vol.
43
,
(
3
)
585
-
596
.
Pickersgill RW, Sumner IG, Collins ME, Warwicker J, Perry B, Bhat KM, Goodenough PW
(
2001
)
.
Modification of the stability of phospholipase A2 by charge engineering
.
FEBS Letters
vol.
281
,
(
1-2
)
219
-
222
.
Lo Leggio L, Kalogiannis S, Eckert K, Teixeira SC, Bhat MK, Andrei C, Pickersgill RW, Larsen S
(
2001
)
.
Substrate specificity and subsite mobility in T. aurantiacus xylanase 10A
.
FEBS Lett
vol.
509
,
(
2
)
303
-
308
.
Pickersgill R, Varvill K, Jones S, Perry B, Fischer B, Henderson I, Garrard S, Sumner I et al.
(
2001
)
.
Making a small enzyme smaller; removing the conserved loop structure of hen lysozyme
.
FEBS Letters
vol.
347
,
(
2-3
)
199
-
202
.
Jenkins J, Pickersgill R
(
2001
)
.
The architecture of parallel beta-helices and related folds
.
PROG BIOPHYS MOL BIO
vol.
77
,
(
2
)
111
-
175
.
Hogg D, Woo EJ, Bolam DN, McKie VA, Gilbert HJ, Pickersgill RW
(
2001
)
.
Crystal structure of mannanase 26A from Pseudomonas cellulosa and analysis of residues involved in substrate binding
.
J Biol Chem
vol.
276
,
(
33
)
31186
-
31192
.
Teixeira S, Lo Leggio L, Pickersgill R, Cardin C
(
2001
)
.
Anisotropic refinement of the structure of Thermoascus aurantiacus xylanase I
.
ACTA CRYSTALLOGR D
vol.
57
,
385
-
392
.
Jenkins J, Mayans O, Smith D, Worboys K, Pickersgill RW
(
2001
)
.
Three-dimensional structure of Erwinia chrysanthemi pectin methylesterase reveals a novel esterase active site
.
J Mol Biol
vol.
305
,
(
4
)
951
-
960
.
Woo EJ, Bauly J, Chen JG, Marshall J, Macdonald H, Lazarus C, Goodenough P, Venis M et al.
(
2000
)
.
Crystallization and preliminary X‐ray analysis of the auxin receptor ABP1
.
Acta Crystallographica Section D, Structural Biology
vol.
56
,
(
11
)
1476
-
1478
.
Woo E-J, Dunwell JM, Goodenough PW, Marvier AC, Pickersgill RW
(
2000
)
.
Germin is a manganese containing homohexamer with oxalate oxidase and superoxide dismutase activities
.
Nature Structural & Molecular Biology
vol.
7
,
(
11
)
1036
-
1040
.
Jenkins J, Leggio LL, Harris G, Pickersgill R
(
2000
)
.
β‐Glucosidase, β‐galactosidase, family A cellulases, family F xylanases and two barley glycanases form a superfamily of enzymes wit 8‐fold β/α architecture and with two conserved glutamates near the carboxy‐terminal ends of β‐strands four and seven
.
FEBS Letters
vol.
362
,
(
3
)
281
-
285
.
Smyth MG, Pickersgill RW, Lax AJ
(
2000
)
.
The potent mitogen Pasteurella multocida toxin is highly resistant to proteolysis but becomes susceptible at lysosomal pH
.
FEBS Letters
vol.
360
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1
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62
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66
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Leggio LL, Jenkins J, Harris GW, Pickersgill RW
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2000
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X‐ray crystallographic study of xylopentaose binding to Pseudomonas fluorescens xylanase A
.
Proteins Structure Function and Bioinformatics
vol.
41
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(
3
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362
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373
.
Katerelos NA, Taylor MAJ, Scott M, Goodenough PW, Pickersgill RW
(
1999
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Crystal structure of a caricain D158E mutant in complex with E‐64
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FEBS Letters
vol.
392
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35
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39
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Leggio LL, Pickersgill RW
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1999
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Xylanase–oligosaccharide interactions studied by a competitive enzyme assay
.
Enzyme and Microbial Technology
vol.
25
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(
8-9
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701
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709
.
Leggio LL, Kalogiannis S, Bhat MK, Pickersgill RW
(
1999
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High resolution structure and sequence of T. aurantiacus Xylanase I: Implications for the evolution of thermostability in family 10 xylanases and enzymes with βα‐barrel architecture
.
Proteins Structure Function and Bioinformatics
vol.
36
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3
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295
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306
.
Aliwan FO, Kroon PA, Faulds CB, Pickersgill R, Williamson G
(
1999
)
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Ferulic acid esterase‐III from Aspergillus niger does not exhibit lipase activity
.
Journal of the Science of Food and Agriculture
vol.
79
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3
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457
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459
.
Scott M, Pickersgill RW, Hazlewood GP, Gilbert HJ, Harris GW
(
1999
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Crystallization and preliminary X‐ray analysis of arabinanase A from Pseudomonas fluorescens subspecies cellulosa
.
Acta Crystallographica Section D, Structural Biology
vol.
55
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2
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544
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546
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Pickersgill R, Scott M, Smith D, Worboys K, Jenkins J
(
1999
)
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Crystallization and preliminary crystallographic analysis of the endo‐polygalacturonase from Erwinia carotovora ssp. carotovora
.
Acta Crystallographica Section D, Structural Biology
vol.
55
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1
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320
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322
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Harris GW, Pickersgill RW, Connerton I, Debeire P, Touzel J, Breton C, Pérez S
(
1998
)
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Structural basis of the properties of an industrially relevant thermophilic xylanase
.
Proteins Structure Function and Bioinformatics
vol.
29
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1
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77
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86
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Woo E-J, Dunwell JM, Goodenough PW, Pickersgill RW
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1998
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Barley oxalate oxidase is a hexameric protein related to seed storage proteins: evidence from X‐ray crystallography
.
FEBS Letters
vol.
437
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1-2
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87
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90
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Pickersgill R, Smith D, Worboys K, Jenkins J
(
1998
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Crystal Structure of Polygalacturonase from Erwinia carotovora ssp. carotovora *
.
Journal of Biological Chemistry
vol.
273
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38
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24660
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24664
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Pickersgill R, Harris G, Leggio LL, Mayans O, Jenkins J
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1998
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Superfamilies: the 4/7 superfamily of βα-barrel glycosidases and the right-handed parallel β-helix superfamily
.
Biochemical Society Transactions
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vol.
26
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197
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Faulds CB, Aliwan FO, de Vries RP, Pickersgill RW, Visser J, Williamson G
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1998
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Chemical and thermal stability of ferulic acid (feruloyl) esterases from Aspergillus
.
Progress in Biotechnology
vol.
15
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41
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46
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Scott M, Pickersgill RW, Hazlewood GP, Bolam D, Gilbert HJ, Harris GW
(
1998
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Crystallization and preliminary X‐ray diffraction studies of a family 26 endo‐β‐1,4 mannanase (ManA) from Pseudomonas fluorescens subspecies cellulosa
.
Acta Crystallographica Section D, Structural Biology
vol.
54
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1
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129
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131
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Jenkins J, Mayans O, Pickersgill R
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1998
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Structure and Evolution of Parallel β-Helix Proteins
.
Journal of Structural Biology
vol.
122
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1-2
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236
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246
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Spurway TD, Morland C, Cooper A, Sumner I, Hazlewood GP, O'Donnell AG, Pickersgill RW, Gilbert HJ
(
1997
)
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Calcium Protects a Mesophilic Xylanase from Proteinase Inactivation and Thermal Unfolding*
.
Journal of Biological Chemistry
vol.
272
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28
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17523
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17530
.
Mayans O, Scott M, Connerton I, Gravesen T, Benen J, Visser J, Pickersgill R, Jenkins J
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1997
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Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases
.
Structure
vol.
5
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5
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677
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689
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Charnock SJ, Lakey JH, Virden R, Hughes N, Sinnott ML, Hazlewood GP, Pickersgill R, Gilbert HJ
(
1997
)
.
Key Residues in Subsite F Play a Critical Role in the Activity of Pseudomonas fluorescens Subspecies cellulosa Xylanase A Against Xylooligosaccharides but Not Against Highly Polymeric Substrates such as Xylan*
.
Journal of Biological Chemistry
vol.
272
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5
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2942
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2951
.
Groves MR, Taylor MA, Scott M, Cummings NJ, Pickersgill RW, Jenkins JA
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1996
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The prosequence of procaricain forms an α-helical domain that prevents access to the substrate-binding cleft
.
Structure
vol.
4
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10
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1193
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Harris GW, Jenkins JA, Connerton I, Cummings N, Leggio LL, Scott M, Hazlewood GP, Laurie JI et al.
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1994
)
.
Structure of the catalytic core of the family F xylanase from Pseudomonas fluorescens and identification of the xylopentaose-binding sites
.
Structure
vol.
2
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11
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1107
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1116
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Taylor MAJ, Baker KC, Connerton IF, Cummings NJ, Harris GW, Henderson IMJ, Jones ST, Pickersgill RW et al.
(
1994
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An unequivocal example of cysteine proteinase activity affected by multiple electrostatic interactions
.
Protein Engineering Design and Selection
vol.
7
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10
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1267
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1276
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Pickersgill R, Jenkins J, Harris G, Nasser W, Robert-Baudouy J
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1994
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The structure of Bacillus subtilis pectate lyase in complex with calcium
.
Nature Structural & Molecular Biology
vol.
1
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10
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717
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723
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Sumner IG, Vaughan A, Eisenthal R, Pickersgill RW, Owen AJ, Goodenough PW
(
1993
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Kinetic analysis of papaya proteinase Ω
.
Biochimica et Biophysica Acta
vol.
1164
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3
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243
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251
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Reveil DF, Cummings NJ, Baker KC, Collins ME, Taylor MAJ, Sumner IG, Pickersgill RW, Connerton IF et al.
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1993
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Nucleotide sequence and expression in Eschericia coli of cDNAs encoding papaya proteinase omega from Carica papaya
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Gene
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127
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2
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221
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225
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Pickersgill RW, Debeire P, Debeire-Gosselin M, Jenkins JA
(
1993
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Crystallization and Preliminary X-ray Analysis of a Thermophilic Bacillus Xylanase
.
Journal of Molecular Biology
vol.
230
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(
2
)
664
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666
.
Golubev AM, Kilimnik AY, Neustroev KN, Pickersgill RW
(
1993
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Crystals of β-Xylanase from Aspergillus oryzae
.
Journal of Molecular Biology
vol.
230
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(
2
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661
-
663
.
Jones ST, Ahlström P, Berendsen HJC, Pickersgill RW
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1993
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Molecular dynamics simulation of a phospholipase A2-substrate complex
.
Biochimica et Biophysica Acta
vol.
1162
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1-2
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135
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142
.
Loris R, Steyaert J, Maes D, Lisgarten J, Pickersgill R, Wyns L
(
1993
)
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Corrections: Crystal Structure Determination and Refinement at 2.3-Å Resolution of the Lentil Lectin (Biochemistry (1993) 32(34) (8772–8781) (10.1021/bi00085a007))
.
Biochemistry
vol.
32
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51
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Pickersgill RW, Jenkins JA, Scott M, Connerton I, Hazlewood GP, Gilbert HJ
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1993
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Crystallization and Preliminary X-ray Analysis of the Catalytic Domain of Xylanase A from Pseudomonas fluorescens subspecies cellulosa
.
Journal of Molecular Biology
vol.
229
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246
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248
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Jenkins J, Nasser W, Scott M, Pickersgill R, Vignon J-C, Robert-Baudouy J
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1992
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Crystallization and preliminary X-ray studies of the pectate lyase from Bacillus subtilis
.
Journal of Molecular Biology
vol.
228
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4
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1255
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1258
.
Loris R, Lisgarten J, Maes D, Pickersgill R, Körber F, Reynolds C, Wyns L
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1992
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Two crystal forms of the lentil lectin diffract to high resolution
.
Journal of Molecular Biology
vol.
223
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2
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579
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581
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Goodenough PW, Bhat KM, Collins ME, Perry BN, Pickersgill RW, Sumner IG, Warwicker J, de Haas GH et al.
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1991
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Changes in activity of porcine phospholipase A2 brought about by charge engineering of a major structural element to alter stability
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Protein Engineering Design and Selection
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4
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929
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Bhat KM, Sumner IG, Perry BN, Collins ME, Pickersgill RW, Goodenough PW
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1991
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A novel method for the purification of porcine phospholipase A2 expressed in E.coli
.
Biochemical and Biophysical Research Communications
vol.
176
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371
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377
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Pickersgill RW, Goodenough PW
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1991
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Enzyme engineering
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Trends in Food Science & Technology
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2
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Pickersgill RW, Sumner IG, Collins ME, Goodenough PW
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1989
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Structural and electrostatic differences between actinidin and papain account for differences in activity
.
Biochemical Journal
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257
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310
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Pickersgill RW
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1988
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A rapid method of calculating charge–charge interaction energies in proteins
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Protein Engineering Design and Selection
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2
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3
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247
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Pickersgill RW, Goodenough PW, Sumner IG, Collins ME
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1988
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The electrostatic fields in the active-site clefts of actinidin and papain
.
Biochemical Journal
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254
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238
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ADAMS MJ, GOVER S, PICKERSGILL RW, HELLIWELL JR
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1983
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.
Structure-function relationships of NAD+- and NADP+-dependent dehydrogenases with particular reference to the three-dimensional structure of 6-phosphogluconate dehydrogenase
.
Biochemical Society Transactions
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11
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429
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435
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Adams MJ, Archibald IG, Bugg CE, Carne A, Gover S, Helliwell JR, Pickersgill RW, White SW
(
1983
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The three dimensional structure of sheep liver 6‐phosphogluconate dehydrogenase at 2.6 A resolution
.
The EMBO Journal
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2
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1009
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1014
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