Publications: Dr Pedro Rodriguez Cutillas

Thompson EM, Patel V, Rajeeve V, Cutillas PR, Stoker AW ( 2022 ) . The cytotoxic action of BCI is not dependent on its stated DUSP1 or DUSP6 targets in neuroblastoma cells . FEBS Open Bio vol. 12 , ( 7 ) 1388 - 1405 .

Grey W, Rio-Machin A, Casado P, Grönroos E, Ali S, Miettinen JJ, Bewicke-Copley F, Parsons A et al. ( 2022 ) . CKS1 inhibition depletes leukemic stem cells and protects healthy hematopoietic stem cells in acute myeloid leukemia . Science translational medicine vol. 14 , ( 650 )

Woodward L, Jones TA, Patel A, Dokal AD, Stone TJ, Rajeeve V, Cutillas PR, Jones DTW et al. ( 2022 ) . LGG-44. Multi-omic analysis reveals integrated signalling networks in paediatric low-grade glioma . Neuro-Oncology vol. 24 , ( Suppl 1 ) i98 - i98 .

Grey W, Rio-Machin A, Casado-Izquierdo P, Gronroos E, Ali S, Miettinen J, Bewicke-Copley F, Parsons A et al. ( 2022 ) . P405: INHIBITION OF CKS1-DEPENDENT PROTEOSTASIS REVEALS VULNERABILITIES IN LEUKAEMIC STEM CELLS WITH CONCOMITANT PROTECTION OF HEALTHY HAEMATOPOIETIC STEM CELLS . HemaSphere vol. 6 , 305 - 306 .

Hijazi M, Casado P, Akhtar N, Alvarez-Teijeiro S, Rajeeve V, Cutillas PR ( 2022 ) . eEF2K Activity Determines Synergy to Cotreatment of Cancer Cells With PI3K and MEK Inhibitors . Mol Cell Proteomics vol. 21 , ( 6 )

Roy-Luzarraga M, Reynolds LE, de Luxán-Delgado B, Maiques O, Wisniewski L, Newport E, Rajeeve V, Drake RJG et al. ( 2022 ) . Suppression of Endothelial Cell FAK Expression Reduces Pancreatic Ductal Adenocarcinoma Metastasis after Gemcitabine Treatment . Cancer Research vol. 82 , ( 10 ) 1909 - 1925 .

Pedicona F, Casado P, Hijazi M, Gribben JG, Rouault-Pierre K, Cutillas PR ( 2022 ) . Targeting the lysine-specific demethylase 1 rewires kinase networks and primes leukemia cells for kinase inhibitor treatment . Science Signaling vol. 15 , ( 730 )

Thorup A-S, Wilson J, Strachan D, Caxaria S, Poulet B, Thomas B, Eldridge S, Nalesso G et al. ( 2022 ) . BLOCKING ROR2 IMPROVES CARTILAGE INTEGRITY AND PROVIDES PAIN RELIEF IN OSTEOARTHRITIS, IN PART BY MODULATING YAP SIGNALLING . Osteoarthritis and Cartilage vol. 30 ,

Newport E, Pedrosa AR, Lees D, Dukinfield M, Carter E, Gomez-Escudero J, Casado P, Rajeeve V et al. ( 2022 ) . Elucidating the role of the kinase activity of endothelial cell focal adhesion kinase in angiocrine signalling and tumour growth . Journal of Pathology vol. 256 , ( 2 ) 235 - 247 .

Murray ER, Menezes S, Henry JC, Williams JL, Alba-Castellón L, Baskaran P, Quétier I, Desai A et al. ( 2022 ) . Disruption of pancreatic stellate cell myofibroblast phenotype promotes pancreatic tumor invasion . Cell Reports vol. 38 , ( 4 )

Casado P, Hijazi M, Gerdes H, Cutillas PR ( 2022 ) . Implementation of Clinical Phosphoproteomics and Proteomics for Personalized Medicine . vol. 2420 ,

Nobre LV, Basanta CC, Pedicona SF, Dokal AD, Arruda A, Smith R, Greenhalgh C, Patella F et al. ( 2021 ) . Abstract LBA013: Phosphoproteomics reveals active drug targets on pathways of resistance and predicts response to midostaurin plus chemotherapy in FLT3 mutant-positive acute myeloid leukemia . Molecular Cancer Therapeutics . vol. 20 , lba013 - lba013 .

Gualtieri A, Kyprianou N, Gregory LC, Vignola ML, Nicholson JG, Tan R, Inoue SI, Scagliotti V et al. ( 2021 ) . Activating mutations in BRAF disrupt the hypothalamo-pituitary axis leading to hypopituitarism in mice and humans . Nature Communications vol. 12 , ( 1 )

Gerdes H, Casado P, Dokal A, Hijazi M, Akhtar N, Osuntola R, Rajeeve V, Fitzgibbon J et al. ( 2021 ) . Drug ranking using machine learning systematically predicts the efficacy of anti-cancer drugs . Nature Communications vol. 12 , ( 1 )

Williams J, paudyal A, Stewart M, Cutillas P, Cox RD, Tinker A, Metherell L ( 2021 ) . Abstract 14037: A Shorter Myosin Light Chain Kinase 3 Isoform Maintains Myosin Light Chain 2 Phosphorylation but Does Not Attenuate the Dilated Cardiomyopathy Seen in C57BL/6N Mice . Circulation vol. 144 , ( Suppl_1 ) a14037 - a14037 .

Khorsandi SE, Dokal AD, Rajeeve V, Britton DJ, Illingworth MS, Heaton N, Cutillas PR ( 2021 ) . Computational Analysis of Cholangiocarcinoma Phosphoproteomes Identifies Patient-Specific Drug Targets . Cancer Res vol. 81 , ( 22 ) 5765 - 5776 .

Lecker LSM, Berlato C, Maniati E, Delaine-Smith R, Pearce OMT, Heath O, Nichols SJ, Trevisan C et al. ( 2021 ) . Tgfbi production by macrophages contributes to an immunosuppressive microenvironment in ovarian cancer . Cancer Research vol. 81 , ( 22 ) 5706 - 5719 .

Veiga Nobre LMV, Pedicona SF, Dokal A, Arruda A, Smith R, Greenhalgh C, Patella F, Casado-Izquierdo P et al. ( 2021 ) . Proteomics and Phospho-Proteomics Reveal Predictive Signatures of Response and Mechanisms of Resistance to Midostaurin Plus Chemotherapy in FLT3 Mutant Positive Acute Myeloid Leukemia . Blood vol. 138 , ( Supplement 1 ) 3462 - 3462 .

Davidson K, Grevitt P, Contreras-Gerenas MF, Bridge KS, Hermida M, Shah KM, Mardakheh FK, Stubbs M et al. ( 2021 ) . Targeted therapy for LIMD1-deficient non-small cell lung cancer subtypes . Cell Death and Disease vol. 12 , ( 11 )

Heydt Q, Xintaropoulou C, Clear A, Austin M, Pislariu I, Miraki-Moud F, Cutillas P, Korfi K et al. ( 2021 ) . Adipocytes disrupt the translational programme of acute lymphoblastic leukaemia to favour tumour survival and persistence . Nature Communications

Heward J, Konali L, D'Avola A, Close K, Yeomans A, Philpott M, Dunford J, Rahim T et al. ( 2021 ) . KDM5 inhibition offers a novel therapeutic strategy for the treatment of KMT2D mutant lymphomas . Blood vol. 138 , ( 5 ) 370 - 381 .

Freire-Benéitez V, Pomella N, Millner TO, Dumas AA, Niklison-Chirou MV, Maniati E, Wang J, Rajeeve V et al. ( 2021 ) . Correction to 'Elucidation of the BMI1 interactome identifies novel regulatory roles in glioblastoma' . NAR Cancer vol. 3 , ( 2 )

Dokal AD, Arruda A, Smith R, Greenhalgh C, Patella F, Casado-Izquierdo PM, Wrench B, Theaker J et al. ( 2021 ) . Predictive phosphoproteomic signatures for midostaurin plus chemotherapy response in FLT3 mutant positive acute myeloid leukaemia . Journal of Clinical Oncology vol. 39 , ( 15_suppl ) 7019 - 7019 .

Freire-Benéitez V, Pomella N, Millner TO, Dumas AA, Niklison-Chirou MV, Maniati E, Wang J, Rajeeve V et al. ( 2021 ) . Elucidation of the BMI1 interactome identifies novel regulatory roles in glioblastoma . NAR Cancer vol. 3 , ( 1 )

Martín-Guerrero SM, Alonso P, Iglesias A, Cimadevila M, Brea J, Loza MI, Casado P, Martín-Oliva D et al. ( 2021 ) . His452Tyr polymorphism in the human 5-HT2A receptor affects clozapine-induced signaling networks revealed by quantitative phosphoproteomics . Biochem Pharmacol vol. 185 ,

Bradley D, Viéitez C, Rajeeve V, Selkrig J, Cutillas PR, Beltrao P ( 2021 ) . Sequence and Structure-Based Analysis of Specificity Determinants in Eukaryotic Protein Kinases . Cell Rep vol. 34 , ( 2 )

Edelmann J, Dokal AD, Vilventhraraja E, Holzmann K, Britton D, Klymenko T, Döhner H, Cragg M et al. ( 2021 ) . Rituximab and obinutuzumab differentially hijack the B-cell receptor and NOTCH1 signaling pathways . iScience102089 - 102089 .

Martín-Guerrero SM, Casado P, Hijazi M, Rajeeve V, Plaza-Díaz J, Abadía-Molina F, Navascués J, Cuadros MA et al. ( 2020 ) . PARP-1 activation after oxidative insult promotes energy stress-dependent phosphorylation of YAP1 and reduces cell viability . Biochem J vol. 477 , ( 23 ) 4491 - 4513 .

Patel A, Jones T, Woodward L, Dokal A, Rajeeve V, Cutillas P, Stone T, Jacques T et al. ( 2020 ) . LGG-57. SIGNALLING MECHANISMS IN PAEDIATRIC LOW-GRADE GLIOMA . Neuro-Oncology vol. 22 , ( Suppl 3 ) iii377 - iii377 .

Patani H, Rushton MD, Higham J, Teijeiro SA, Oxley D, Cutillas P, Sproul D, Ficz G ( 2020 ) . Transition to naïve human pluripotency mirrors pan-cancer DNA hypermethylation . Nature Communications vol. 11 , ( 1 )

Rio-Machin A, Casado-Izquierdo P, Miettinen J, Bewicke-Copley F, Khan N, Demeulemeester J, Krizsán S, Zheng J et al. ( 2020 ) . Integration of Deep Multi-Omics Profiling Veals New Insights into the Biology of Poor-Risk Acute Myeloid Leukemia . Blood vol. 136 , ( Supplement 1 ) 39 - 40 .

Fernández-Varo G, Perramón M, Carvajal S, Oró D, Casals E, Boix L, Oller L, Macías-Muñoz L et al. ( 2020 ) . Bespoken Nanoceria: An Effective Treatment in Experimental Hepatocellular Carcinoma . Hepatology vol. 72 , ( 4 ) 1267 - 1282 .

Xu R, Jones W, Wilcz-Villega E, Costa ASH, Rajeeve V, Bentham RB, Bryson K, Nagano A et al. ( 2020 ) . The breast cancer oncogene IKKε coordinates mitochondrial function and serine metabolism . EMBO Reports vol. 21 , ( 9 )

Akhtar N, Waleed Baig M, Haq IU, Rajeeve V, Rodriguez Cutillas P ( 2020 ) . Withanolide metabolites inhibit PI3K/AKT and MAPK pro-survival pathways and induce apoptosis in acute myeloid leukemia cells . Biomedicines vol. 8 , ( 9 )

Gadaleta E, Fourgoux P, Pirró S, Thorn GJ, Nelan R, Ironside A, Rajeeve V, Cutillas PR et al. ( 2020 ) . Characterization of four subtypes in morphologically normal tissue excised proximal and distal to breast cancer . npj Breast Cancer vol. 6 , ( 1 )

Gadaleta E, Fourgoux P, Pirró S, Thorn GJ, Nelan R, Ironside A, Rajeeve V, Cutillas PR et al. ( 2020 ) . Characterization of four subtypes in morphologically normal tissue excised proximal and distal to breast cancer . NPJ Breast Cancer vol. 6 , ( 1 )

Kostaras E, Kaserer T, Lazaro G, Heuss SF, Hussain A, Casado P, Hayes A, Yandim C et al. ( 2020 ) . A systematic molecular and pharmacologic evaluation of AKT inhibitors reveals new insight into their biological activity . British Journal of Cancer vol. 123 , ( 4 ) 542 - 555 .

Wong PP, Muñoz-Félix JM, Hijazi M, Kim H, Robinson SD, De Luxán-Delgado B, Rodríguez-Hernández I, Maiques O et al. ( 2020 ) . Cancer Burden Is Controlled by Mural Cell-β3-Integrin Regulated Crosstalk with Tumor Cells . Cell vol. 181 , ( 6 ) 1346 - 1363.e21 .

Invergo BM, Petursson B, Akhtar N, Bradley D, Giudice G, Hijazi M, Cutillas P, Petsalaki E et al. ( 2020 ) . Prediction of Signed Protein Kinase Regulatory Circuits . Cell Syst vol. 10 , ( 5 ) 384 - 396.e9 .

Demircioglu F, Wang J, Candido J, Costa ASH, Casado P, de Luxan Delgado B, Reynolds LE, Gomez-Escudero J et al. ( 2020 ) . Cancer associated fibroblast FAK regulates malignant cell metabolism . Nature Communications vol. 11 , ( 1 ) 1290 - 1290 .

Huang KW, Reebye V, Czysz K, Ciriello S, Dorman S, Reccia I, Lai HS, Peng L et al. ( 2020 ) . Liver Activation of Hepatocellular Nuclear Factor-4α by Small Activating RNA Rescues Dyslipidemia and Improves Metabolic Profile . Molecular Therapy - Nucleic Acids vol. 19 , 361 - 370 .

Hijazi M, Smith R, Rajeeve V, Bessant C, Cutillas PR ( 2020 ) . Reconstructing kinase network topologies from phosphoproteomics data reveals cancer-associated rewiring . Nature Biotechnology

Cutillas PR, Hijazi M, Smith R, Bessant C, Britton D ( 2019 ) . Abstract C088: Chemical phosphoproteomics systematically identifies circuitries of kinase networks in cancer cells and predicts their response to kinase inhibitors . Molecular Cancer Therapeutics . vol. 18 , c088 - c088 .

Carvajal S, Perramón M, Casals G, Oró D, Ribera J, Morales-Ruiz M, Casals E, Casado P et al. ( 2019 ) . Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes . Int J Mol Sci vol. 20 , ( 23 )

Grey W, Casado-Izquierdo P, Cutillas P, Bonnet D ( 2019 ) . Combination Therapy Targeting CKS1-Dependent Protein Degradation Reduces AML Burden Whilst Protecting Normal Haematopoietic Stem Cellsfrom Cytarabine Toxicity . Blood vol. 134 , ( Supplement_1 ) 2535 - 2535 .

Edelmann J, Dokal A, Holzmann K, Britton DJ, Vilventhraraja E, Smith RJ, Cragg MS, Braun A et al. ( 2019 ) . Rituximab and Obinutuzumab Induce Direct B-Cell Death Via B-Cell Receptor (BCR) Signaling, but Rituximab Elicits Stronger BCR-Derived Pro-Survival Signals Diminishing Apoptosis . Blood vol. 134 , ( Supplement_1 ) 1579 - 1579 .

Martín-Guerrero SM, Casado P, Muñoz-Gámez JA, Carrasco M-C, Navascués J, Cuadros MA, López-Giménez JF, Cutillas PR et al. ( 2019 ) . Poly(ADP-Ribose) Polymerase-1 inhibition potentiates cell death and phosphorylation of DNA damage response proteins in oxidative stressed retinal cells . Exp Eye Res vol. 188 ,

Thomas JR, Moore KM, Sproat C, Mo S, Wolanska KI, Maldonado H, Haider S, Newman D et al. ( 2019 ) . Integrin alpha V beta 6-EGFR crosstalk regulates bidirectional force transmission and controls breast cancer invasion . INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY . vol. 100 , A39 - A39 .

Alrawashdeh W, Jones R, Dumartin L, Radon TP, Cutillas PR, Feakins RM, Dmitrovic B, Demir IE et al. ( 2019 ) . Perineural invasion in pancreatic cancer: proteomic analysis and in vitro modelling . Molecular Oncology vol. 13 , ( 5 ) 1075 - 1091 .

Kostelnik KB, Barker A, Schultz C, Mitchell TP, Rajeeve V, White IJ, Aurrand-Lions M, Nourshargh S et al. ( 2019 ) . Dynamic trafficking and turnover of JAM-C is essential for endothelial cell migration . PLoS Biology vol. 17 , ( 12 )

Naba A, Pearce OMT, Del Rosario A, Ma D, Ding H, Rajeeve V, Cutillas PR, Balkwill FR et al. ( 2018 ) . Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics . Journal of Proteome Research vol. 16 , ( 8 ) 3083 - 3091 .

Ferrandiz N, Barroso C, Telecan O, Shao N, Kim HM, Testori S, Faull P, Cutillas P et al. ( 2018 ) . Erratum to: Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. elegans oocyte meiosis (Nature Communications, (2018), 9, 1, (834), 10.1038/s41467-018-03229-5) . Nature Communications vol. 9 , ( 1 )

Ferrandiz N, Barroso C, Telecan O, Shao N, Kim HM, Testori S, Faull P, Cutillas P et al. ( 2018 ) . Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. Elegans oocyte meiosis . Nature Communications vol. 9 , ( 1 )

Edelmann J, Britton DJ, Vilventhraraja E, Dokal A, Holzmann K, Cragg MC, Braun A, Cutillas P et al. ( 2018 ) . Anti-CD20 Monoclonal Antibodies Hijack the B-Cell Receptor Signaling Cascade Thereby Activating the NOTCH1 Signaling Pathway . Blood . Conference: American Society of Hematology Annual Congress vol. 132 , 588 - 588 .

Arnandis T, Monteiro P, Adams SD, Bridgeman VL, Rajeeve V, Gadaleta E, Marzec J, Chelala C et al. ( 2018 ) . Oxidative Stress in Cells with Extra Centrosomes Drives Non-Cell-Autonomous Invasion . Developmental Cell vol. 47 , ( 4 ) 409 - 424.e9 .

Angulo-Urarte A, Casado P, Castillo SD, Kobialka P, Kotini MP, Figueiredo AM, Castel P, Rajeeve V et al. ( 2018 ) . Endothelial cell rearrangements during vascular patterning require PI3-kinase-mediated inhibition of actomyosin contractility . Nat Commun vol. 9 , ( 1 )

Álvarez-Teijeiro S, García-Inclán C, Villaronga MÁ, Casado P, Hermida-Prado F, Granda-Díaz R, Rodrigo JP, Calvo F et al. ( 2018 ) . Factors Secreted by Cancer-Associated Fibroblasts that Sustain Cancer Stem Properties in Head and Neck Squamous Carcinoma Cells as Potential Therapeutic Targets . Cancers (Basel) vol. 10 , ( 9 )

Simon T, Pinioti S, Schellenberger P, Rajeeve V, Wendler F, Cutillas PR, King A, Stebbing J et al. ( 2018 ) . Shedding of bevacizumab in tumour cells-derived extracellular vesicles as a new therapeutic escape mechanism in glioblastoma . Mol Cancer vol. 17 , ( 1 )

Casado P, Wilkes EH, Miraki-Moud F, Hadi MM, Rio-Machin A, Rajeeve V, Pike R, Iqbal S et al. ( 2018 ) . Proteomic and genomic integration identifies kinase and differentiation determinants of kinase inhibitor sensitivity in leukemia cells . Leukemia vol. 32 , ( 8 ) 1818 - 1822 .

Tummala H, Dokal AD, Walne A, Ellison A, Cardoso S, Amirthasigamanipillai S, Kirwan M, Browne I et al. ( 2018 ) . Genome instability is a consequence of transcription deficiency in patients with bone marrow failure harboring biallelic ERCC6L2 variants . Proc Natl Acad Sci U S A vol. 115 , ( 30 ) 7777 - 7782 .

Reebye V, Huang KW, Lin V, Jarvis S, Cutilas P, Dorman S, Ciriello S, Andrikakou P et al. ( 2018 ) . Gene activation of CEBPA using saRNA: Preclinical studies of the first in human saRNA drug candidate for liver cancer . Oncogene vol. 37 , ( 24 ) 3216 - 3228 .

Corominas MP, Restoy SC, Casals G, Oró D, Ribera J, Casals E, Casado-Izquierdo P, Varo GF et al. ( 2018 ) . THU-028 Cerium oxide nanoparticles protect against oxidant mediated injury and recover kinase activity of multiple pathways in human-derived hepatocellular carcinoma cells . Journal of Hepatology . vol. 68 ,

Fearon AE, Carter EP, Clayton NS, Wilkes EH, Baker AM, Kapitonova E, Bakhouche BA, Tanner Y et al. ( 2018 ) . PHLDA1 Mediates Drug Resistance in Receptor Tyrosine Kinase-Driven Cancer . Cell Reports vol. 22 , ( 9 ) 2469 - 2481 .

Casado P, Wilkes EH, Miraki-Moud F, Hadi MM, Rio-Machin A, Rajeeve V, Pike R, Iqbal S et al. ( 2017 ) . Proteomic and genomic integration identifies kinase and differentiation determinants of kinase inhibitor sensitivity in leukemia cells . Leukemia

Pearce OMT, Delaine-Smith R, Maniati E, Nichols S, Wang J, Böhm S, Rajeeve V, Ullah D et al. ( 2017 ) . Deconstruction of a metastatic tumor microenvironment reveals a common matrix response in human cancers . Cancer Discov

Kyprianou N, Gregory L, Vignola ML, Marinelli E, Gualtieri A, Scagliotti V, Davis S, Casado P et al. ( 2017 ) . The MAPK effector B-Raf is essential for hypothalamic-pituitary axis development and activating mutations in BRAF Cause Congenital hypopituitarism . Endocrine Abstracts

Dermit M, Dokal A, Cutillas PR ( 2017 ) . Approaches to identify kinase dependencies in cancer signalling networks . FEBS Lett vol. 591 , ( 17 ) 2577 - 2592 .

Wilkes EH, Casado P, Rajeeve V, Cutillas PR ( 2017 ) . Kinase activity ranking using phosphoproteomics data (KARP) quantifies the contribution of protein kinases to the regulation of cell viability . Mol Cell Proteomics vol. 16 , ( 9 ) 1694 - 1704 .

Cutillas PR ( 2017 ) . Systems Biology of Oncogenic Signaling . MOLECULAR & CELLULAR PROTEOMICS . vol. 16 , S15 - S15 .

Wilkes E, Cutillas PR ( 2017 ) . Label-Free Phosphoproteomic Approach for Kinase Signaling Analysis . Methods Mol Biol vol. 1636 , 199 - 217 .

Yuzugulen J, Douthwaite JA, Wood EG, Villar IC, Patel NSA, Jegard J, Gaertner H, Rossitto-Borlat I et al. ( 2017 ) . Characterisation of preproendothelin-1 derived peptides identifies Endothelin-Like Domain Peptide as a modulator of Endothelin-1 . Sci Rep vol. 7 , ( 1 ) 4956 - 4956 .

Casado-Izquierdo P, Wilkes EH, Miraki-Moud F, Hadi MM, Rio-Machin A, Rajeeve V, Pike R, Sameena I et al. ( 2017 ) . PHOSPHOPROTEOMICS AND MASS CYTOMETRY SIGNATURES OF PRIMARY AML CELL DIFFERENTIATION ARE ASSOCIATED WITH SENSITIVITY TO KINASE INHIBITORS . HAEMATOLOGICA . vol. 102 , 203 - 203 .

Fernandez-Varo G, Oro D, Yudina T, Carvajal S, Marfa S, Boix L, Perramon M, Oller L et al. ( 2017 ) . Cerium oxide nanoparticles display anti-tumoral activity and improve survival in experimental hepatocellular carcinoma . JOURNAL OF HEPATOLOGY . Conference: The International Liver Congress™ EASL 2017 - European Association for the Study of the Live ( Amsterdam, Holland ) from: 19/04/2017 to: 23/04/2017 , vol. 66 , S229 - S230 .

Cutillas PR ( 2017 ) . Targeted In-Depth Quantification of Signaling Using Label-Free Mass Spectrometry . Methods Enzymol vol. 585 , 245 - 268 .

Graupera M, Angulo-Urarte A, Kobialka P, Castel P, Casado PM, Affolter M, Gerhardt H, Belting H-G et al. ( 2017 ) . The p110alpha/PI3K controls endothelial cell rearrangements during angiogenesis by stimulating MLC2 dephosphorylation . JOURNAL OF VASCULAR RESEARCH . vol. 54 , 73 - 73 .

Dermit M, Casado P, Rajeeve V, Wilkes EH, Foxler DE, Campbell H, Critchlow S, Sharp TV et al. ( 2016 ) . Oxidative stress downstream of mTORC1 but not AKT causes a proliferative defect in cancer cells resistant to PI3K inhibition . Oncogene

Afzal TA, Luong LA, Chen D, Zhang C, Yang F, Chen Q, An W, Wilkes E et al. ( 2016 ) . NCK Associated Protein 1 Modulated by miRNA-214 Determines Vascular Smooth Muscle Cell Migration, Proliferation, and Neointima Hyperplasia . Journal of the American Heart Association vol. 5 , ( 12 ) e004629 - e004629 .

Casado P, Wilkes E, Hadi MM, Rajeeve V, Miraki-Moud F, Pike R, Del Rio-Machin A, Iqbal S et al. ( 2016 ) . Differentiation Status Revealed By Shotgun Phosphoproteomics Determines Sensitivity of Primary AML Cells to Kinase Inhibitors . Blood . vol. 128 , 840 - 840 .

Britton DJ, Wilkes E, Casado P, Rajeeve V, Fitzgibbon J, Gribben J, Cutillas PR ( 2016 ) . Proteomic Analysis Directs Effective Drug Selection in Relapsed AML By Quantifying Drug Targets . Blood . vol. 128 , 5265 - 5265 .

Villoria MT, Ramos F, Dueñas E, Faull P, Cutillas PR, Clemente-Blanco A ( 2016 ) . Stabilization of the metaphase spindle by Cdc14 is required for recombinational DNA repair . EMBO J vol. 36 , ( 1 ) 79 - 101 .

Prysyazhna O, Scotcher J, Boguslavskyi A, Kistamas K, Hadgraft N, Martin ED, Worthington J, Rudyk O et al. ( 2016 ) . 348 Redox Control of Protein Kinase G Iα Fine-Tunes the Frank-Starling Law of the Heart in Vivo by Regulating Diastolic Relaxation . Free Radical Biology and Medicine . vol. 100 ,

Scotcher J, Prysyazhna O, Boguslavskyi A, Kistamas K, Hadgraft N, Martin ED, Worthington J, Rudyk O et al. ( 2016 ) . Disulfide-activated protein kinase G Iα regulates cardiac diastolic relaxation and fine-tunes the Frank-Starling response . Nature Communications vol. 7 , 13187 - 13187 .

Casado P, Hijazi M, Britton D, Cutillas PR ( 2016 ) . Impact of phosphoproteomics in the translation of kinase targeted therapies . Proteomics

Sciacovelli M, Gonçalves E, Johnson TI, Zecchini VR, da Costa ASH, Gaude E, Drubbel AV, Theobald SJ et al. ( 2016 ) . Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition . Nature vol. 537 , ( 7621 ) 544 - 547 .

Bouchekioua-Bouzaghou K, Lenihan C, Shia A, Wilkes E, Casado-Izquierdo P, Cutillas P, Schmid P ( 2016 ) . Abstract 2819: Characterization of the mechanisms of early and later stages of resistance to the selective CDK4/6 inhibitor palbociclib . Cancer Research . vol. 76 , 2819 - 2819 .

Montfort A, Pearce OMT, Maniati E, Vincent B, Bixby LM, Bo hm S, Dowe T, Wilkes EH et al. ( 2016 ) . A strong B cell response is part of the immune landscape in human high-grade serous ovarian metastases . Clinical Cancer Research

Kulbe H, Iorio F, Chakravarty P, Milagre CS, Moore R, Thompson RG, Everitt G, Canosa M et al. ( 2016 ) . Integrated transcriptomic and proteomic analysis identifies protein kinase CK2 as a key signaling node in an inflammatory cytokine network in ovarian cancer cells . Oncotarget vol. 7 , ( 13 ) 15648 - 15661 .

Nadarajan S, Mohideen F, Tzur YB, Ferrandiz N, Crawley O, Montoya A, Faull P, Snijders AP et al. ( 2016 ) . The MAP kinase pathway coordinates crossover designation with disassembly of synaptonemal complex proteins during meiosis . eLife vol. 5 , ( FEBRUARY2016 )

Lenihan C, Bouchekioua-Bouzaghou K, Shia A, Wilkes E, Casado-Izquierdo1 P, Cutillas P, Schmid P ( 2016 ) . Abstract P3-06-02: Characterization of resistance to the selective CDK4/6 inhibitor palbociclib in ER positive breast cancer . Cancer Research . vol. 76 ,

Quétier I, Marshall JJT, Spencer-Dene B, Lachmann S, Casamassima A, Franco C, Escuin S, Worrall JT et al. ( 2016 ) . Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion . Cell Reports vol. 14 , ( 3 ) 440 - 448 .

Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Arozena AA, Adachi H, Adams CM et al. ( 2016 ) . Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) . Autophagy vol. 12 , ( 1 ) 1 - 222 .

Cutillas PR, Crnogorac-Jurcevic T ( 2016 ) . Application of proteomics in cancer biomarker discovery: GeLC-MS/MS . vol. 1381 , 201 - 209 .

Arnandis T, Rajeeve V, Brennan CH, Cutillas PR, Godinho SA ( 2016 ) . Paracrine-mediated invasion induced by centrosome amplification . MOLECULAR BIOLOGY OF THE CELL . vol. 27 ,

Tomlinson JAP, Caplin B, Boruc O, Bruce-Cobbold C, Cutillas P, Dormann D, Faull P, Grossman RC et al. ( 2015 ) . Reduced Renal Methylarginine Metabolism Protects against Progressive Kidney Damage . J Am Soc Nephrol vol. 26 , ( 12 ) 3045 - 3059 .

Terfve CDA, Wilkes EH, Casado P, Cutillas PR, Saez-Rodriguez J ( 2015 ) . Large-scale models of signal propagation in human cells derived from discovery phosphoproteomic data . Nat Commun vol. 6 ,

Carretero L, Llavona P, López-Hernández A, Casado P, Cutillas PR, de la Peña P, Barros F, Domínguez P ( 2015 ) . ERK and RSK are necessary for TRH-induced inhibition of r-ERG potassium currents in rat pituitary GH3 cells . Cell Signal vol. 27 , ( 9 ) 1720 - 1730 .

Herranz N, Gallage S, Mellone M, Wuestefeld T, Klotz S, Hanley CJ, Raguz S, Acosta JC et al. ( 2015 ) . mTOR regulates MAPKAPK2 translation to control the senescence-associated secretory phenotype . Nat Cell Biol vol. 17 , ( 9 ) 1205 - 1217 .

Wilkes EH, Terfve C, Gribben JG, Saez-Rodriguez J, Cutillas PR ( 2015 ) . Empirical inference of circuitry and plasticity in a kinase signaling network . Proceedings of the National Academy of Sciences of the United States of America vol. 112 , ( 25 ) 7719 - 7724 .

Cutillas PR ( 2015 ) . Role of phosphoproteomics in the development of personalized cancer therapies . Proteomics Clin Appl vol. 9 , ( 3-4 ) 383 - 395 .

Rotival M, Ko J-H, Srivastava PK, Kerloc'h A, Montoya A, Mauro C, Faull P, Cutillas PR et al. ( 2015 ) . Integrating phosphoproteome and transcriptome reveals new determinants of macrophage multinucleation . Mol Cell Proteomics vol. 14 , ( 3 ) 484 - 498 .

Hall AM, Vilasi A, Garcia-Perez I, Lapsley M, Alston CL, Pitceathly RDS, McFarland R, Schaefer AM et al. ( 2015 ) . The urinary proteome and metabonome differ from normal in adults with mitochondrial disease . Kidney Int vol. 87 , ( 3 ) 610 - 622 .

Wilkes EH, Casado P, Cutillas PR ( 2014 ) . Approaches for measuring signalling plasticity in the context of resistance to targeted cancer therapies . Biochem Soc Trans vol. 42 , ( 4 ) 791 - 797 .

Rajeeve V, Vendrell I, Wilkes E, Torbett N, Cutillas PR ( 2014 ) . Cross-species proteomics reveals specific modulation of signaling in cancer and stromal cells by phosphoinositide 3-kinase (PI3K) inhibitors . Mol Cell Proteomics vol. 13 , ( 6 ) 1457 - 1470 .

Casado P, Bilanges B, Rajeeve V, Vanhaesebroeck B, Cutillas PR ( 2014 ) . Environmental Stress Affects the Activity of Metabolic and Growth Factor Signaling Networks and Induces Autophagy Markers in MCF7 Breast Cancer Cells . MOLECULAR & CELLULAR PROTEOMICS vol. 13 , ( 3 ) 836 - 848 .

Yuzugulen J, Wood EG, Villar IC, Douthwaite JA, Patel NSAI, Jegard J, Montoya A, Cutillas P et al. ( 2013 ) . Characterisation of the “Endothelin-Like Domain Peptide” (ELDP) co-synthesised with Endothelin-1 from the EDN1 gene . Life Sciences vol. 93 , ( 25-26 )

Lumb S, Torbett N, Vendrell I, Turner H, Page M, Hales P, Maloney A, Vanhaesebroeck B et al. ( 2013 ) . THU0045 Epratuzumab, an Antibody Targeting CD22 on B Cells, Induces Phosphoprotein Changes Following B-Cell Receptor Activation in Vitro . Annals of the Rheumatic Diseases vol. 72 , ( Suppl 3 )

Casado P, Alcolea MP, Iorio F, Rodríguez-Prados J-C, Vanhaesebroeck B, Saez-Rodriguez J, Joel S, Cutillas PR ( 2013 ) . Phosphoproteomics data classify hematological cancer cell lines according to tumor type and sensitivity to kinase inhibitors . Genome Biol vol. 14 , ( 4 )

Casado P, Rodriguez-Prados J-C, Cosulich SC, Guichard S, Vanhaesebroeck B, Joel S, Cutillas PR ( 2013 ) . Kinase-substrate enrichment analysis provides insights into the heterogeneity of signaling pathway activation in leukemia cells . Sci Signal vol. 6 , ( 268 )

Rajeeve V, Pearce W, Cascante M, Vanhaesebroeck B, Cutillas PR ( 2013 ) . Polyamine production is downstream and upstream of oncogenic PI3K signalling and contributes to tumour cell growth . Biochem J vol. 450 , ( 3 ) 619 - 628 .

Alrawashdeh W, Jones R, Cutillas P, Wijesuriya N, Crnogorac-Jurcevic T ( 2013 ) . Proteomic analysis of perineural invasion in pancreatic adenocarcinoma reveals up-regulation of neurosecretory protein VGF in invaded nerves . Pancreatology vol. 13 , ( 2 )

Beltran L, Casado P, Rodríguez-Prados J-C, Cutillas PR ( 2012 ) . Global profiling of protein kinase activities in cancer cells by mass spectrometry . J Proteomics vol. 77 , 492 - 503 .

Butler T, Montoya A, Clear AJ, Coutinho R, Taussig DC, Cutillas P, Gribben JG ( 2012 ) . Anergic Response to B-Cell Receptor Signaling in Chronic Lymphocytic Leukemia: The Differing Roles of IgD and IgM in the Microenvironment and Peripheral Blood . Blood . vol. 120 , 2889 - 2889 .

Beltran L, Cutillas PR ( 2012 ) . Advances in phosphopeptide enrichment techniques for phosphoproteomics . Amino Acids vol. 43 , ( 3 ) 1009 - 1024 .

Cappadona S, Baker PR, Cutillas PR, Heck AJR, van Breukelen B ( 2012 ) . Current challenges in software solutions for mass spectrometry-based quantitative proteomics . AMINO ACIDS vol. 43 , ( 3 ) 1087 - 1108 .

Alcolea MP, Casado P, Rodríguez-Prados J-C, Vanhaesebroeck B, Cutillas PR ( 2012 ) . Phosphoproteomic analysis of leukemia cells under basal and drug-treated conditions identifies markers of kinase pathway activation and mechanisms of resistance . Mol Cell Proteomics vol. 11 , ( 8 ) 453 - 466 .

Kitromilidou C, McDonald D, Cutillas P, Ghazaly E, Perry J, Csaba B, Joel S, Powles T ( 2012 ) . Abstract 1792: The potential role of targeting HER1-4 in bladder cancer . Cancer Research vol. 72 , ( 8_Supplement ) 1792 - 1792 .

Kulbe H, Chakravarty P, Moore R, Iorio F, Montoya A, Saez-Rodriguez J, Cutillas P, Balkwill F ( 2012 ) . Abstract 49: Targeting the TNF network in human ovarian cancer - A systems biology approach . Cancer Research vol. 72 , ( 8_Supplement ) 49 - 49 .

Burling KA, Cutillas PR, Church D, Lapsley M, Norden AGW ( 2012 ) . Analysis of molecular forms of urine Retinol-Binding Protein in Fanconi Syndrome and design of an accurate immunoassay . CLINICA CHIMICA ACTA vol. 413 , ( 3-4 ) 483 - 489 .

Worthington J, Cutillas PR, Timms JF ( 2011 ) . IMAC/TiO2 enrich for peptide modifications other than phosphorylation: Implications for chromatographic choice and database searching in phosphoproteomics . PROTEOMICS vol. 11 , ( 23 ) 4583 - 4587 .

Beltran L, Chaussade C, Vanhaesebroeck B, Cutillas PR ( 2011 ) . Calpain interacts with class IA phosphoinositide 3-kinases regulating their stability and signaling activity . Proc Natl Acad Sci U S A vol. 108 , ( 39 ) 16217 - 16222 .

Montoya A, Beltran L, Casado P, Rodríguez-Prados J-C, Cutillas PR ( 2011 ) . Characterization of a TiO₂ enrichment method for label-free quantitative phosphoproteomics . Methods vol. 54 , ( 4 ) 370 - 378 .

Cutillas PR, Jørgensen C ( 2011 ) . Biological signalling activity measurements using mass spectrometry . Biochem J vol. 434 , ( 2 ) 189 - 199 .

Casado P, Cutillas PR ( 2011 ) . A self-validating quantitative mass spectrometry method for assessing the accuracy of high-content phosphoproteomic experiments . Mol Cell Proteomics vol. 10 , ( 1 )

Jueliger S, Maharaj L, Iyengar S, Ghazaly E, Cutillas P, Auer R, Rommel C, Li L et al. ( 2010 ) . Abstract 4489: The activity of novel, potent, selective PI3K/mTOR pathway inhibitors in B-cell malignancies . Cancer Research . vol. 70 , 4489 - 4489 .

Cutillas PR ( 2010 ) . Analysis of peptides in biological fluids by LC-MS/MS . Methods Mol Biol vol. 658 , 311 - 321 .

Cutillas PR, Timms JF ( 2010 ) . Approaches and applications of quantitative LC-MS for proteomics and activitomics . Methods Mol Biol vol. 658 , 3 - 17 .

Alcolea MP, Cutillas PR ( 2010 ) . In-depth analysis of protein phosphorylation by multidimensional ion exchange chromatography and mass spectrometry . Methods Mol Biol vol. 658 , 111 - 126 .

Timms JF, Cutillas PR ( 2010 ) . Overview of quantitative LC-MS techniques for proteomics and activitomics . vol. 658 ,

Alcolea MP, Cutillas PR ( 2010 ) . Quantification of protein kinase activities by LC-MS . Methods Mol Biol vol. 658 , 325 - 337 .

Cutillas PR ( 2009 ) . Quantifying oncogenic signalling by mass spectrometry . AMINO ACIDS vol. 37 , ( 4 ) 762 - 762 .

Alcolea MP, Kleiner O, Cutillas PR ( 2009 ) . Increased confidence in large-scale phosphoproteomics data by complementary mass spectrometric techniques and matching of phosphopeptide data sets . J Proteome Res vol. 8 , ( 8 ) 3808 - 3815 .

Quintana LF, Campistol JM, Alcolea MP, Banon-Maneus E, Sole-Gonzalez A, Cutillas PR ( 2009 ) . Application of Label-free Quantitative Peptidomics for the Identification of Urinary Biomarkers of Kidney Chronic Allograft Dysfunction . MOL CELL PROTEOMICS vol. 8 , ( 7 ) 1658 - 1673 .

Vilasi A, Cutillas PR, Unwin RJ ( 2008 ) . Application of proteomic techniques to the study of urine and renal tissue . PROTEOM CLIN APPL vol. 2 , ( 12 ) 1564 - 1574 .

Graupera M, Guillermet-Guibert J, Foukas LC, Phng LK, Cain RJ, Salpekar A, Pearce W, Meek S et al. ( 2008 ) . Angiogenesis selectively requires the p110 alpha isoform of PI3K to control endothelial cell migration . NATURE vol. 453 , ( 7195 ) 662 - U9 .

Cutillas PR, Vanhaesebroeck B ( 2007 ) . Quantitative profile of five murine core proteomes using label-free functional proteomics . Mol Cell Proteomics vol. 6 , ( 9 ) 1560 - 1573 .

Vilasi A, Cutillas PR, Maher AD, Zirah SFM, Capasso G, Norden AWG, Holmes E, Nicholson JK et al. ( 2007 ) . Combined proteomic and metabonomic studies in three genetic forms of the renal Fanconi syndrome . Am J Physiol Renal Physiol vol. 293 , ( 2 ) F456 - F467 .

Cutillas PR, Vanhaesebroeck B ( 2007 ) . Profiling protein expression by label-free quantitative mass Spectrometry . MOL CELL PROTEOMICS vol. 6 , ( 8 ) 14 - 14 .

Geering B, Cutillas PR, Nock G, Gharbi SI, Vanhaesebroeck B ( 2007 ) . Class IA phosphoinositide 3-kinases are obligate p85-p110 heterodimers . Proc Natl Acad Sci U S A vol. 104 , ( 19 ) 7809 - 7814 .

VANHAESEBROECK BAM, Gharbi SL, Nock G, Geering B, Cutillas PR ( 2007 ) . Class IA phosphoinositide 3-kinases are obligate p85-p110 heterodimers . Proc Natl Acad Sci U S A vol. 104 , 7809 - 7814 .

Norden AGW, Rodriguez-Cutillas P, Unwin Lindmans RJ ( 2007 ) . Erratum: Clinical urinary peptidomics: Learning to walk before we can run (Clinical Chemistry (2007) 53, (375-376) DOI: 10.1373/clinchem.2006.084038) . Clinical Chemistry vol. 53 , ( 4 )

Geering B, Cutillas PR, Vanhaesebroeck B ( 2007 ) . Regulation of class IA PI3Ks: is there a role for monomeric PI3K subunits? . Biochem Soc Trans . vol. 35 , 199 - 203 .

Norden AGW, Rodriguez-Cutillas P, Unwin RJ ( 2007 ) . Clinical urinary peptidomics: Learning to walk before we can run . Clinical Chemistry vol. 53 , ( 3 ) 375 - 376 .

CUTILLAS PR, Vanhaesebroeck BAM, Khwaja A, Waterfield M, Gharbi S, Pearce W, Graupera M ( 2006 ) . Ultrasensitive and absolute quantification of the phosphoinositide 3-kinase/Akt signal transduction pathway by mass spectrometry . Proc Natl Acad Sci USA vol. 103 , ( 24 ) 8959 - 8964 .

Cutillas PR, Geering B, Waterfield MD, Vanhaesebroeck B ( 2005 ) . Quantification of gel-separated proteins and their phosphorylation sites by LC-MS using unlabeled internal standards: analysis of phosphoprotein dynamics in a B cell lymphoma cell line . Mol Cell Proteomics vol. 4 , ( 8 ) 1038 - 1051 .

CUTILLAS PR, Biber J, Marks J, Waterfield M, Cramer R, Stieger B, Jacob R, Burlingame AL et al. ( 2005 ) . Proteomic analysis of plasma membrane vesicles isolated from the rat renal cortex . Proteomics vol. 5 , ( 1 ) 101 - 112 .

Norden AGW, Sharratt P, Cutillas PR, Cramer R, Gardner SC, Unwin RJ ( 2004 ) . Quantitative amino acid and proteomic analysis: very low excretion of polypeptides >750 Da in normal urine . Kidney Int . vol. 66 , 1994 - 2003 .

CUTILLAS PR, Burlingame AL, Waterfield MD, Norden AG, Cramer R, Hansen KC, Chalkley RJ, Unwin RJ ( 2004 ) . The urinary proteome in Fanconi syndrome implies specificity in the reabsorption of proteins by renal proximal tubule cells . Am J Physiol Renal Physiol vol. 287 , ( 3 ) F353 - F364 .

Cutillas P, Burlingame A, Unwin R ( 2004 ) . Proteomic strategies and their application in studies of renal function . News Physiol Sci vol. 19 , 114 - 119 .

Cutillas PR, Norden AG, Cramer R, Burlingame AL, Unwin RJ ( 2004 ) . Urinary proteomics of renal Fanconi syndrome . Contrib Nephrol vol. 141 , 155 - 169 .

Cutillas PR, Norden AGW, Cramer R, Burlingame AL, Unwin RJ ( 2003 ) . Detection and analysis of urinary peptides by on-line liquid chromatography and mass spectrometry: application to patients with renal Fanconi syndrome . Clin Sci (Lond) vol. 104 , ( 5 ) 483 - 490 .