Skip to main content
Research

Publications: Dr Ewan Main

Perez-Riba A, Komives E, Main E, Itzhaki L ( 2019 ) . Decoupling a tandem-repeat protein: impact of multiple loop insertions on a modular scaffold . Scientific Reports
Wright JN, Wong WL, Harvey JA, Garnett JA, Itzhaki LS, Main ERG ( 2019 ) . Scalable Geometrically Designed Protein Cages Assembled via Genetically Encoded Split Inteins . Structure vol. 27 , ( 5 ) 776 - 784.e4 .
Perez-Riba A, Lowe A, MAIN E, Itzhaki L ( 2018 ) . Context-dependent energetics of loop extensions in a family of tandem-repeat proteins . Biophysical Journal
Harvey JA, Itzhaki LS, Main ERG ( 2018 ) . Programmed Protein Self-Assembly Driven by Genetically Encoded Intein-Mediated Native Chemical Ligation . ACS Synth Biol vol. 7 , ( 4 ) 1067 - 1074 .
Lowe AR, Perez-Riba A, Itzhaki LS, Main ERG ( 2018 ) . PyFolding: Open-Source Graphing, Simulation, and Analysis of the Biophysical Properties of Proteins . Biophys J vol. 114 , ( 3 ) 516 - 521 .
Perez-Riba A, Lowe A, Main E, Komives E, Itzhaki L ( 2017 ) . Entropically-Challenged Tandem-repeat Proteins: Breakdown of Nearest-Neighbour Cooperativity . PROTEIN SCIENCE . vol. 26 , 108 - 108 .
Millership C, Phillips JJ, Main ERG ( 2016 ) . Ising Model Reprogramming of a Repeat Protein's Equilibrium Unfolding Pathway . J Mol Biol vol. 428 , ( 9 Pt A ) 1804 - 1817 .
Perez-Riba A, Main ER, Itzhaki LS ( 2016 ) . The Effect of Loop Insertions on the Folding of Tandem-Repeat Proteins . Biophysical Journal . vol. 110 ,
Rowling PJE, Sivertsson EM, Perez-Riba A, Main ERG, Itzhaki LS ( 2015 ) . Dissecting and reprogramming the folding and assembly of tandem-repeat proteins . Biochemical Society Transactions vol. 43 , 881 - 888 .
Main ERG, Phillips JJ, Millership C ( 2013 ) . Repeat protein engineering: creating functional nanostructures/biomaterials from modular building blocks . Biochem Soc Trans vol. 41 , ( 5 ) 1152 - 1158 .
Singh SK, Boyle AL, Main ERG ( 2013 ) . LcrH, a class II chaperone from the type three secretion system, has a highly flexible native structure . J Biol Chem vol. 288 , ( 6 ) 4048 - 4055 .
Phillips JJ, Millership C, Main ERG ( 2012 ) . Fibrous nanostructures from the self-assembly of designed repeat protein modules . Angew Chem Int Ed Engl vol. 51 , ( 52 ) 13132 - 13135 .
Phillips JJ, Javadi Y, Millership C, Main ERG ( 2012 ) . Modulation of the multistate folding of designed TPR proteins through intrinsic and extrinsic factors . Protein Sci vol. 21 , ( 3 ) 327 - 338 .
Mahendrarajah K, Dalby PA, Wilkinson B, Jackson SE, Main ERG ( 2011 ) . A high-throughput fluorescence chemical denaturation assay as a general screen for protein-ligand binding . Anal Biochem vol. 411 , ( 1 ) 155 - 157 .
Boyd LK, Mercer B, Thompson D, Main E, Watts FZ ( 2010 ) . Characterisation of the SUMO-like domains of Schizosaccharomyces pombe Rad60 . PLoS One vol. 5 , ( 9 )
Gaudet M, Remtulla N, Jackson SE, Main ERG, Bracewell DG, Aeppli G, Dalby PA ( 2010 ) . Protein denaturation and protein:drugs interactions from intrinsic protein fluorescence measurements at the nanolitre scale . PROTEIN SCI vol. 19 , ( 8 ) 1544 - 1554 .
Javadi Y, Main ERG ( 2009 ) . Exploring the folding energy landscape of a series of designed consensus tetratricopeptide repeat proteins . P NATL ACAD SCI USA vol. 106 , ( 41 ) 17383 - 17388 .
Main ERG, Lowe AR, Mochrie SGJ, Jackson SE, Regan L ( 2005 ) . A recurring theme in protein engineering: the design, stability and folding of repeat proteins . CURR OPIN STRUC BIOL vol. 15 , ( 4 ) 464 - 471 .
Kajander T, Cortajarena AL, Main ERG, Mochrie SGJ, Regan L ( 2005 ) . A new folding paradigm for repeat proteins . J AM CHEM SOC vol. 127 , ( 29 ) 10188 - 10190 .
Main ERG, Stott K, Jackson SE, Regan L ( 2005 ) . Local and long-range stability in tandemly arrayed tetratricopeptide repeats . P NATL ACAD SCI USA vol. 102 , ( 16 ) 5721 - 5726 .
Maxwell KL, Wildes D, Zarrine-Afsar A, de los Rios MA, Brown AG, Friel CT, Hedberg L, Horng JC et al. ( 2005 ) . Protein folding: Defining a "standard" set of experimental conditions and a preliminary kinetic data set of two-state proteins . PROTEIN SCI vol. 14 , ( 3 ) 602 - 616 .
Main ERG, Jackson SE, Regan L ( 2003 ) . The folding and design of repeat proteins: reaching a consensus . CURR OPIN STRUC BIOL vol. 13 , ( 4 ) 482 - 489 .
Main ERG, Xiong Y, Cocco MJ, D'Andrea L, Regan L ( 2003 ) . Design of stable alpha-helical arrays from an idealized TPR motif . STRUCTURE vol. 11 , ( 5 ) 497 - 508 .
Main ERG, Fulton KF, Daggett V, Jackson SE ( 2001 ) . A comparison of experimental and computational methods for mapping the interactions present in the transition state for folding of FKBP12 . J BIOL PHYS vol. 27 , ( 2 ) 99 - 117 .
Main ERG, Jackson SE ( 1999 ) . Does trifluoroethanol affect folding pathways and can it be used as a probe of structure in transition states? . NAT STRUCT BIOL vol. 6 , ( 9 ) 831 - 835 .
Main ERG, Fulton KF, Jackson SE ( 1999 ) . Folding pathway of FKBP12 and characterisation of the transition state . J MOL BIOL vol. 291 , ( 2 ) 429 - 444 .
Fulton KF, Main ERG, Daggett V, Jackson SE ( 1999 ) . Mapping the interactions present in the transition state for unfolding/folding of FKBP12 . J MOL BIOL vol. 291 , ( 2 ) 445 - 461 .
Main ERG, Fulton KF, Jackson SE ( 1998 ) . Context-dependent nature of destabilizing mutations on the stability of FKBP12 . BIOCHEMISTRY-US vol. 37 , ( 17 ) 6145 - 6153 .