Publications: Prof John Connelly

Anderson CJ, Talmane L, Luft J, Connelly J, Nicholson MD, Verburg JC, Pich O, Campbell S et al. ( 2024 ) . Strand-resolved mutagenicity of DNA damage and repair . Nature vol. 630 , ( 8017 ) 744 - 751 .

Ross R, Guo Y, Walker RN, Bergamaschi D, Shaw TJ, Connelly JT ( 2024 ) . Biomechanical Activation of Keloid Fibroblasts Promotes Lysosomal Remodeling and Exocytosis . Journal of Investigative Dermatology vol. 144 , ( 12 ) 2730 - 2741 .

Millar FR, Pennycuick A, Muir M, Quintanilla A, Hari P, Freyer E, Gautier P, Meynert A et al. ( 2022 ) . Toll-like receptor 2 orchestrates a tumor suppressor response in non-small cell lung cancer . Cell Reports vol. 41 , ( 6 )

Jones CFE, Di Cio S, Connelly JT, Gautrot JE ( 2022 ) . Design of an Integrated Microvascularized Human Skin-on-a-Chip Tissue Equivalent Model . Frontiers in Bioengineering and Biotechnology vol. 10 ,

Sarmin AM, Moussaid NE, Suntornnond R, Tyler EJ, Kim Y-H, Di Cio S, Megone WV, Pearce O et al. ( 2022 ) . Multi-Scale Analysis of the Composition, Structure, and Function of Decellularized Extracellular Matrix for Human Skin and Wound Healing Models . Biomolecules vol. 12 , ( 6 )

Sarmin AM, Connelly JT ( 2022 ) . Fabrication of Human Skin Equivalents Using Decellularized Extracellular Matrix . Current Protocols vol. 2 , ( 3 )

Zhang Y, Hollis D, Ross R, Snow T, Terrill NJ, Lu Y, Wang W, Connelly J et al. ( 2022 ) . Investigating the Fibrillar Ultrastructure and Mechanics in Keloid Scars Using In Situ Synchrotron X-ray Nanomechanical Imaging . Materials (Basel) vol. 15 , ( 5 )

Pundel OJ, Blowes LM, Connelly JT ( 2022 ) . Extracellular Adhesive Cues Physically Define Nucleolar Structure and Function . Advanced Science vol. 9 , ( 10 )

Hannen R, Connelly J, Myers S, Ojeh N ( 2022 ) . Skin tissue engineering and keratinocyte stem cell therapy . Tissue Engineering, Third Edition ,

Connelly JT, Gavara N, Sliogeryte K, Blowes LM ( 2021 ) . Research Techniques Made Simple: Analysis of Skin Cell and Tissue Mechanics Using Atomic Force Microscopy . Journal of Investigative Dermatology vol. 141 , ( 8 ) 1867 - 1871.e1 .

Singh B, Abdelgawad ME, Ali Z, Bailey J, Budyn E, Civita P, Clift MJD, Connelly JT et al. ( 2021 ) . Towards More Predictive, Physiological and Animal-free In Vitro Models: Advances in Cell and Tissue Culture 2020 Conference Proceedings . Alternatives to Laboratory Animals vol. 49 , ( 3 ) 93 - 110 .

Wu Y, Fortunato GM, Okesola BO, Brocchetti FLPD, Suntornnond R, Connelly J, De Maria C, Rodriguez-Cabello JC et al. ( 2021 ) . An interfacial self-assembling bioink for the manufacturing of capillary-like structures with tuneable and anisotropic permeability . Biofabrication vol. 13 , ( 3 )

Costa P, Blowes LM, Laly AC, Connelly JT ( 2021 ) . Regulation of collective cell polarity and migration using dynamically adhesive micropatterned substrates . Acta Biomaterialia vol. 126 , 291 - 300 .

Laly AC, Sliogeryte K, Pundel OJ, Ross R, Keeling MC, Avisetti D, Waseem A, Gavara N et al. ( 2021 ) . The keratin network of intermediate filaments regulates keratinocyte rigidity sensing and nuclear mechanotransduction . Science Advances vol. 7 , ( 5 )

Di Cio S, Iskratsch T, Connelly JT, Gautrot JE ( 2019 ) . Contractile myosin rings and cofilin-mediated actin disassembly orchestrate ECM nanotopography sensing . Biomaterials vol. 232 ,

Uttagomol J, Ahmad US, Rehman A, Huang Y, Laly AC, Kang A, Soetaert J, Chance R et al. ( 2019 ) . Evidence for the Desmosomal Cadherin Desmoglein-3 in Regulating YAP and Phospho-YAP in Keratinocyte Responses to Mechanical Forces . International Journal of Molecular Sciences vol. 20 , ( 24 )

Blowes LM, Sliogeryte K, Gavara N, Connelly J ( 2019 ) . 268 Geometry-dependent chromatin remodelling in keratinocytes is altered by cell-cell adhesions . Journal of Investigative Dermatology . vol. 139 ,

Almeida FV, Gammon L, Laly AC, Pundel OJ, Bishop CL, Connelly JT ( 2019 ) . High‐Content Analysis of Cell Migration Dynamics within a Micropatterned Screening Platform . Advanced Biosystems1900011 - 1900011 .

Connelly JT ( 2019 ) . Chapter One Biophysical regulation of epidermal fate and function . Epidermal Stem Cell Niche , vol. 3 , Elsevier

Ghetti M, Topouzi H, Theocharidis G, Papa V, Williams G, Bondioli E, Cenacchi G, Connelly JT et al. ( 2018 ) . Subpopulations of dermal skin fibroblasts secrete distinct extracellular matrix: implications for using skin substitutes in the clinic . British Journal of Dermatology vol. 179 , ( 2 ) 381 - 393 .

Kenny FN, Drymoussi Z, Delaine-Smith R, Kao AP, Laly AC, Knight MM, Philpott MP, Connelly JT ( 2018 ) . Tissue stiffening promotes keratinocyte proliferation through activation of epidermal growth factor signaling . Journal of Cell Science vol. 131 , ( 10 ) Article jcs215780 , 215780 - 215780 .

Li D, Sharili AS, Connelly J, Gautrot JE ( 2018 ) . Highly Stable RNA Capture by Dense Cationic Polymer Brushes for the Design of Cytocompatible, Serum-Stable SiRNA Delivery Vectors . Biomacromolecules

Pearce OMT, Delaine-Smith R, Maniati E, Nichols S, Wang J, Böhm S, Rajeeve V, Ullah D et al. ( 2017 ) . Deconstruction of a metastatic tumor microenvironment reveals a common matrix response in human cancers . Cancer Discov

Theocharidis G, Connelly JT ( 2017 ) . Minor collagens of the skin with not so minor functions . Journal of Anatomy vol. 235 , ( 2 ) 418 - 429 .

GAUTROT JEE, Di Cio S, Boggild T, Connelly J, Sutherland DS ( 2016 ) . Differential Integrin Expression Regulates Cell Sensing of the Matrix Nanoscale Geometry . Acta Biomaterialia

Kanapathy M, Hachach‐Haram N, Bystrzonowski N, Connelly JT, O'Toole EA, Becker DL, Mosahebi A, Richards T ( 2016 ) . Epidermal grafting for wound healing: a review on the harvesting systems, the ultrastructure of the graft and the mechanism of wound healing . International Wound Journal vol. 14 , ( 1 ) 16 - 23 .

Sharili AS, Kenny FN, Vartiainen MK, Connelly JT ( 2016 ) . Nuclear actin modulates cell motility via transcriptional regulation of adhesive and cytoskeletal genes . Sci Rep vol. 6 , 33893 - 33893 .

Kao AP, Connelly JT, Barber AH ( 2016 ) . 3D nanomechanical evaluations of dermal structures in skin . Journal of the mechanical behavior of biomedical materials vol. 57 , 14 - 23 .

Wongkaew N, Connelly JT, Baeumner AJ ( 2016 ) . Taking advantage of liposomes in microfluidic systems . Liposomes in Analytical Methodologies ,

Theocharidis G, Drymoussi Z, Kao AP, Barber AH, Lee DA, Braun KM, Connelly JT ( 2016 ) . Type VI Collagen Regulates Dermal Matrix Assembly and Fibroblast Motility . J Invest Dermatol vol. 136 , ( 1 ) 74 - 83 .

Almeida FV, Walko G, McMillan JR, McGrath JA, Wiche G, Barber AH, Connelly JT ( 2015 ) . The cytolinker plectin regulates nuclear mechanotransduction in keratinocytes . Journal of Cell Science vol. 128 , ( 24 ) 4475 - 4486 .

Dalbay MT, Thorpe SD, Connelly JT, Chapple JP, Knight MM ( 2015 ) . Adipogenic Differentiation of hMSCs is Mediated by Recruitment of IGF‐1r Onto the Primary Cilium Associated With Cilia Elongation . Stem Cells vol. 33 , ( 6 ) 1952 - 1961 .

Kenny FN, Connelly JT ( 2014 ) . Integrin-mediated adhesion and mechano-sensing in cutaneous wound healing . Cell and Tissue Research vol. 360 , ( 3 ) 571 - 582 .

Costa P, Gautrot JE, Connelly JT ( 2014 ) . Directing cell migration using micropatterned and dynamically adhesive polymer brushes . Acta Biomater vol. 10 , ( 6 ) 2415 - 2422 .

Sharili AS, Connelly JT ( 2014 ) . Nucleocytoplasmic shuttling: a common theme in mechanotransduction . Biochem Soc Trans vol. 42 , ( 3 ) 645 - 649 .

McMurray RJ, Wann AKT, Thompson CL, Connelly JT, Knight MM ( 2013 ) . Surface topography regulates wnt signaling through control of primary cilia structure in mesenchymal stem cells . Sci Rep vol. 3 ,

Tan DW, Jensen KB, Trotter MW, Connelly JT, Broad S, Watt FM ( 2013 ) . Single-cell gene expression profiling reveals functional heterogeneity of undifferentiated human epidermal cells . Development vol. 140 , ( 7 ) 1433 - 1444 .

Hosny NA, Song M, Connelly JT, Ameer-Beg S, Knight MM, Wheeler AP ( 2013 ) . Super-resolution imaging strategies for cell biologists using a spinning disk microscope . PLoS One vol. 8 , ( 10 )

Costa P, Almeida FVM, Connelly JT ( 2012 ) . Biophysical signals controlling cell fate decisions: how do stem cells really feel? . Int J Biochem Cell Biol vol. 44 , ( 12 ) 2233 - 2237 .

Trappmann B, Gautrot JE, Connelly JT, Strange DGT, Li Y, Oyen ML, Cohen Stuart MA, Boehm H et al. ( 2012 ) . Extracellular-matrix tethering regulates stem-cell fate . Nat Mater vol. 11 , ( 7 ) 642 - 649 .

Driskell RR, Juneja VR, Connelly JT, Kretzschmar K, Tan DW-M, Watt FM ( 2012 ) . Clonal growth of dermal papilla cells in hydrogels reveals intrinsic differences between Sox2-positive and -negative cells in vitro and in vivo . J Invest Dermatol vol. 132 , ( 4 ) 1084 - 1093 .

Trappmann B, Gautrot JE, Connelly JT, Strange DGT, Li Y, Oyen ML, Cohen Stuart MA, Boehm H et al. ( 2012 ) . Erratum: Extracellular-matrix tethering regulates stem-cell fate (Nature Materials (2012) 11 (642-649)) . Nature Materials vol. 11 , ( 8 )

Trappmann B, Gautrot JE, Connelly JT, Strange DGT, Li Y, Oyen ML, Cohen Stuart MA, Boehm H et al. ( 2012 ) . Extracellular-matrix tethering regulates stem-cell fate . Nature Materials vol. 11 , ( 7 ) 642 - 649 .

Connelly JT ( 2012 ) . Terminal differentiation of human epidermal stem cells on micro-patterned substrates . Methods Mol Biol vol. 916 , 15 - 22 .

Connelly JT, Petrie TA, García AJ, Levenston ME ( 2011 ) . Fibronectin- and collagen-mimetic ligands regulate bone marrow stromal cell chondrogenesis in three-dimensional hydrogels . Eur Cell Mater vol. 22 , 168 - 176 .

Connelly JT, Mishra A, Gautrot JE, Watt FM ( 2011 ) . Shape-induced terminal differentiation of human epidermal stem cells requires p38 and is regulated by histone acetylation . PLoS One vol. 6 , ( 11 )

Connelly JT, Gautrot JE, Trappmann B, Tan DW-M, Donati G, Huck WTS, Watt FM ( 2010 ) . Actin and serum response factor transduce physical cues from the microenvironment to regulate epidermal stem cell fate decisions . Nat Cell Biol vol. 12 , ( 7 ) 711 - 718 .

Connelly JT, Vanderploeg EJ, Mouw JK, Wilson CG, Levenston ME ( 2010 ) . Tensile loading modulates bone marrow stromal cell differentiation and the development of engineered fibrocartilage constructs . Tissue Eng Part A vol. 16 , ( 6 ) 1913 - 1923 .

Gautrot JE, Trappmann B, Oceguera-Yanez F, Connelly J, He X, Watt FM, Huck WTS ( 2010 ) . Exploiting the superior protein resistance of polymer brushes to control single cell adhesion and polarisation at the micron scale . Biomaterials vol. 31 , ( 18 ) 5030 - 5041 .

Kondapalli S, Connelly JT, Baeumner AJ, Kirby BJ ( 2009 ) . On-chip electrophoretic concentration of liposomes for antibody-based viral biosensors . Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences . 612 - 614 .

Connelly JT, Levenston ME ( 2008 ) . Integrin ligand density regulates cytoskeletal organization and the differentiation of mesenchymal progenitors in RGD-modified agarose hydrogels . 8th World Biomaterials Congress 2008 . vol. 1 ,

Connelly JT, García AJ, Levenston ME ( 2008 ) . Interactions between integrin ligand density and cytoskeletal integrity regulate BMSC chondrogenesis . J Cell Physiol vol. 217 , ( 1 ) 145 - 154 .

Connelly JT, Wilson CG, Levenston ME ( 2008 ) . Characterization of proteoglycan production and processing by chondrocytes and BMSCs in tissue engineered constructs . Osteoarthritis Cartilage vol. 16 , ( 9 ) 1092 - 1100 .

Nugen SR, Asiello PJ, Connelly JT, Baeumner AJ ( 2008 ) . PMMA biosensor for nucleic acids with integrated mixer and electrochemical detection . 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference . 937 - 939 .

Mouw JK, Connelly JT, Wilson CG, Michael KE, Levenston ME ( 2007 ) . Dynamic compression regulates the expression and synthesis of chondrocyte-specific matrix molecules in bone marrow stromal cells . Stem Cells vol. 25 , ( 3 ) 655 - 663 .

Connelly JT, García AJ, Levenston ME ( 2007 ) . Inhibition of in vitro chondrogenesis in RGD-modified three-dimensional alginate gels . Biomaterials vol. 28 , ( 6 ) 1071 - 1083 .

Connelly JT, Vanderploeg EJ, Levenston ME ( 2004 ) . The influence of cyclic tension amplitude on chondrocyte matrix synthesis: experimental and finite element analyses . Biorheology vol. 41 , ( 3-4 ) 377 - 387 .